2020
Al-Shayeb, Basem; Sachdeva, Rohan; Chen, Lin-Xing; Ward, Fred; Munk, Patrick; Devoto, Audra; Castelle, Cindy J.; Olm, Matthew R.; Bouma-Gregson, Keith; Amano, Yuki; He, Christine; Méheust, Raphaël; Brooks, Brandon; Thomas, Alex; Lavy, Adi; Matheus-Carnevali, Paula; Sun, Christine; Goltsman, Daniela S. A.; Borton, Mikayla A.; Sharrar, Allison; Jaffe, Alexander L.; Nelson, Tara C.; Kantor, Rose; Keren, Ray; Lane, Katherine R.; Farag, Ibrahim F.; Lei, Shufei; Finstad, Kari; Amundson, Ronald; Anantharaman, Karthik; Zhou, Jinglie; Probst, Alexander J.; Power, Mary E.; Tringe, Susannah G.; Li, Wen-Jun; Wrighton, Kelly; Harrison, Sue; Morowitz, Michael; Relman, David A.; Doudna, Jennifer A.; Lehours, Anne-Catherine; Warren, Lesley; Cate, Jamie H. D.; Santini, Joanne M.; Banfield, Jillian F.
Clades of huge phages from across Earth’s ecosystems Journal Article
In: Nature, vol. 578, pp. 425-431, 2020.
Abstract | Links | BibTeX | Tags: bacteriophage, ERCZO, metagenomes, metagenomic sequencing
@article{Al-Shayeb2020,
title = {Clades of huge phages from across Earth’s ecosystems},
author = {Basem Al-Shayeb and Rohan Sachdeva and Lin-Xing Chen and Fred Ward and Patrick Munk and Audra Devoto and Cindy J. Castelle and Matthew R. Olm and Keith Bouma-Gregson and Yuki Amano and Christine He and Raphaël Méheust and Brandon Brooks and Alex Thomas and Adi Lavy and Paula Matheus-Carnevali and Christine Sun and Daniela S. A. Goltsman and Mikayla A. Borton and Allison Sharrar and Alexander L. Jaffe and Tara C. Nelson and Rose Kantor and Ray Keren and Katherine R. Lane and Ibrahim F. Farag and Shufei Lei and Kari Finstad and Ronald Amundson and Karthik Anantharaman and Jinglie Zhou and Alexander J. Probst and Mary E. Power and Susannah G. Tringe and Wen-Jun Li and Kelly Wrighton and Sue Harrison and Michael Morowitz and David A. Relman and Jennifer A. Doudna and Anne-Catherine Lehours and Lesley Warren and Jamie H. D. Cate and Joanne M. Santini and Jillian F. Banfield},
doi = {10.1038/s41586-020-2007-4},
year = {2020},
date = {2020-02-12},
journal = {Nature},
volume = {578},
pages = {425-431},
abstract = {Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of 735 kb, which is—to our knowledge—the largest phage genome to be described to date. Thirty-five genomes were manually curated to completion (circular and no gaps). Expanded genetic repertoires include diverse and previously undescribed CRISPR–Cas systems, transfer RNAs (tRNAs), tRNA synthetases, tRNA-modification enzymes, translation-initiation and elongation factors, and ribosomal proteins. The CRISPR–Cas systems of phages have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. In addition, some phages may repurpose bacterial CRISPR–Cas systems to eliminate competing phages. We phylogenetically define the major clades of huge phages from human and other animal microbiomes, as well as from oceans, lakes, sediments, soils and the built environment. We conclude that the large gene inventories of huge phages reflect a conserved biological strategy, and that the phages are distributed across a broad bacterial host range and across Earth’s ecosystems.},
keywords = {bacteriophage, ERCZO, metagenomes, metagenomic sequencing},
pubstate = {published},
tppubtype = {article}
}